Journal: bioRxiv
Article Title: On the mode of anti- C. albicans activity of a bis(benzoxaborole) analogue of Tavaborole
doi: 10.1101/2024.07.29.605614
Figure Lengend Snippet: Candida albicans metabolic activity reduction by benzoxaborole derivatives Influence of bezoxaboroles concentrations on metabolic activity of blastoconidial cells. MTS test (3- (4.5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS, Promega, USA) was used to assess C. albicans proliferation. ( A ) The suspension of C. albicans cells in medium YEPD at the density of 1-3 x 10 2 CFU/mL was incubated with compounds 2 and Tavaborole at the range of conc.: 0.25-256 μg/mL for 48 h at 37 ° C. ( B ) The activity of benzoxaboroles against C. albicans growing in a medium with exogenous ergosterol (exErg) at 400 µg/mL for 48 h. %viable cells = OD490 test sample/OD490 control x 100%, control means cells treated with 0.96% DMSO. The results from 3 independent experiments were analyzed. Statistically significant differences were noted between non-treated growth and treated samples (P< 0.05, Unpaired t-test).
Article Snippet: [ ] Appropriately, after 10-fold dilution series 100 μL aliquots from microtiter plates were used to inoculate YEPD agar plates (A&A Biotechnology, Poland) and after 48h incubation at 37°C the grown colonies were counted.
Techniques: Activity Assay, Suspension, Incubation, Control